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??̳??ҳ>>????????>>6??ҩ?????ϸ??ɸѡʵ??

??1ҳ??ҳ??һҳ1??һҳβҳ
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?????ڣ?2018-07-14 19:00
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6??ҩ?????ϸ??ɸѡʵ??

6????Ȼ??ȡ???????ϸ??ɸѡʵ?飬Ԥ??9?????????п????ṩ????ģ?????ϵQQ1020788318




ʵ????Ŀ
??Ʒ??????
ʱ??
????????ģ??????????(IC50 )

ϸ????SRA01/04

ҩ?H2O2

Ũ?ȣ?0??100??200??300??500??750??1000??mol/L

6h

12h

24h

48h
????????ģ??????????(ROS)

ϸ????SRA01/04

ҩ?H2O2

Ũ?ȣ?0??100??200??300??500??750??1000??mol/L 

6h

12h

24h

48h



??һ??

ҩ?6??  

1.control

2.H2O2

3.ҩ?1 ??M??+ H2O2

4.ҩ?10 ??M??+ H2O2

5.ҩ?100 ??M??+H2O2

2-5??ϸ???ȼ???H2O2????T1????ģ

3-5????ҩ?????24h,48h,72h??

24h

48h

72h
CCK-8???
ϸ??IC50




?ڶ???

ҩ?6??

1. control

2. H2O2

3.ҩ?Ũ??1??+ H2O2

4.ҩ?Ũ??2??+ H2O2

2-4??ϸ???ȼ???H2O2????T1????ģ

3-4????ҩ?????24h,48h,72h??

24h

48h

72h
SOD???
ϸ??SOD??????⣨?Լ??У?

ROS???
??ʽ???ROS????

qPCR???
??ȡϸ????RNA??QPCR???SIRT1??Ա?????????-actinΪ?ڲ?

WB???
Western blot???SIRT1??AMPK????p-AMPK??????3??ָ?꣬??-actinΪ?ڲ?



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